Reddit Reddit reviews CRISPR-Cas: A Laboratory Manual

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Science & Math
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Biological Sciences
Biochemistry
CRISPR-Cas: A Laboratory Manual
Cold Spring Harbor Laboratory Pr
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1 Reddit comment about CRISPR-Cas: A Laboratory Manual:

u/MutantCRISPRbaby ยท 2 pointsr/CRISPR

Go to your state University's library. It should be open to the public.

Read the fundamentals:

https://www.nature.com/articles/nprot.2013.143

And


https://www.amazon.com/CRISPR-Cas-Laboratory-Manual-Jennifer-Doudna/dp/1621821315


There is much, much to learn besides the basics of how crispr actually works. Everything from knowing which types of buffer to use, types of water to use, pH of buffers, salt concentrations needed for reactions, counter balance ions, types of gels......becoming a proficient molecular biologist is a skill that take years to develop. I can't build a car or a house if instead some books on the basics of car mechanic repair or carpentry, right? No, you also need knowledge and a lot of hands on experience to become a proficient carpenter. All of the videos etc are nice, but there are sooooooo many more things to think about when actually doing this stuff in the lab in order to get quality results and to avoid things like contamination, low efficiency, and non-reproducible results.