Reddit reviews Laboratory Burner

We found 1 Reddit comments about Laboratory Burner. Here are the top ones, ranked by their Reddit score.

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1 Reddit comment about Laboratory Burner:

u/dansyr · 3 pointsr/orchids

Oops sorry I should have explained better. You don't actually pass your biological stuff through / over flame, just use flame to help maintain a clean workspace. Glass, tools, etc. gets flamed though.

TL;DR: Generic microbiology aseptic technique: article and video

Preface: I did NOT invent this. This is just how we microbiologists do our normal lab work, so it's what I tried to do when I started tissue culturing, and it seems to work just as well for TC. But I suspect this might seem like heresy to a real TC person...

Basic process is:

generously spray workspace (~1.5-2 ft of bench) with 70% ethanol (or 10% bleach if you're into that. I'm an ethanol guy)
Turn on your bunsen burner or this kind of thing as soon as possible after ethanol-ing but ONLY after the ethanol has dried sufficiently to not burn your house down.
Make sure it's on pretty high (you want a fairly loud, roaring blue flame cone that doesn't flicker). The little ethanol-wick burners that I've seen don't seem to generate enough heat for this. Sure, it's enough to flame-sterilize a tool, but it doesn't put out enough heat fast enough to keep up a solid updraft.
Do all your work within a ~6-inch radius around the burner, preferably less. This is the super important part. You want to have your protocorms / the sterile ends of tools / anything sterile to be in that zone. Like, don't burn yourself. But try to live right on the edge. Basically, the flame creates an updraft, keeping things from falling out of the air into your jar, onto your forceps, etc. But the updraft strength falls away fairly fast the further away you get from the flame. Especially since you ethanol'd your bench, there's practically insignificant contaminant contribution from below, so you have a "practically" sterile airspace. Definitely not fully sterile, but practically. And then you basically play the odds game to get your work done.

Tips/techniques for winning the odds game:
Always keep things covered when not directly reaching in. E.g., lid of donor vessel off, reach in, pick protocorms, pull out, lid goes back on, lid of receiving vessel off, place protocorms, pull out, lid goes on. This is the most important, I think: chances of contamination are directly proportional to time the lid is uncovered. The flame helps, but it only works if you have flame + quick & precise work. Which is totally feasible since the actual reaching in and collecting only takes ~2 seconds, moving between the two takes ~1 s, placing takes ~1-2 s.
Don't open things by completely removing the lid unless absolutely necessary, just barely crack the lid enough to reach your tool inside. Sometimes you need to fully open it and set the lid down (inside up), which is OK. Just be quick and close to the flame!
When opening lids, angle lids when open to keep as much of the vessel covered, and have the angle open towards the flame, not you.
With jars set up like yours, briefly tilt the jar and flame the mouths of the jars as soon as you open them to create excurrent air. Not enough to get the whole thing so hot it cooks the life, just enough to heat up the glass at the opening a little bit. A good way to minimize open time - open lid and tilt mouth into flame quickly, pass the opening through once or twice. Then set the jar back down, and set the lid on top (don't screw it on, just set it since nothing will get in with the lid resting shut, especially since you flamed the opening). Now you can just crack the lid open with your left hand, don't need to re-flame since the lip should still be hot from before. And just reflame before every transfer (or every other transfer).
No air currents
Try not to breathe or breathe very softly when you have anything open
Flame your forceps/spatula often, and flame it VERY hot. Usually I flame the working end of mine so hot that if the temperature equilibrated on it, I'd have very burned fingertips. But, then I squirt some 70% ethanol on the working end to cool it down. This isn't super safe, and try not to breathe the smoke/fumes that happens, but it saves a ton of time from having to balance the forceps somewhere in the sterile space to cool. And, of course, wait for the EtOH to evaporate before handling the biology.
Ethanol gloves before you start.

EDIT: Formatting. And I'm so sorry this got so long... I got carried away