Best media bottles for labs according to redditors
We found 23 Reddit comments discussing the best media bottles for labs. We ranked the 13 resulting products by number of redditors who mentioned them. Here are the top 20.
We found 23 Reddit comments discussing the best media bottles for labs. We ranked the 13 resulting products by number of redditors who mentioned them. Here are the top 20.
The larger jars are about $250 each on amazon.
Pyrex Media Bottles, 10,000mL with GL-45 Screw Cap https://www.amazon.com/dp/B0055V2VME/ref=cm_sw_r_cp_api_i_OF3UDbZFE7MCP
Amazon! You can find packs of 12/24/48/etc. vials / amber bottles for extremely cheap. You can also get very cheap (in a good way, they're all high quality) nasal sprayers, as well. I have SOOOO many left over amber bottles and such.
Here are some examples!
1oz Amber Bottles 12pk
Same thing, but 2oz.
12 Amber Vials with Droppers
Larger Clear Round Pyrex bottle
2ml amber dram bottles (these also come in 24-36+ counts, too
...and there's so much more! Amazon is a goldmine for research materials in my experience! Hope I could help!
I think bottletop is the way to go, I've been doing it with syringe filters and shit but man it fucking sucks and it just doesn't feel like it's very sterile sometimes atleast for a newbie like myself. Used bottletop today to brew up some Test Cyp and holy shit was it way easier and just felt a lot more sterile. Used the following stuffs
https://www.amazon.com/ABN-Brake-Bleeder-Vacuum-Pump/dp/B005BT90DI/ref=sr_1_2?s=automotive&ie=UTF8&qid=1475218483&sr=1-2&keywords=hand+brake+pump
https://www.amazon.com/PYREX-250mL-Round-Storage-Bottles/dp/B004XR5TIO/
https://www.amazon.com/Autofil-1151-RLS-Bottle-Filtration-micrometer/dp/B00HSDSRHU
Going to be brewing some more test cyp/tren a/mast p/test p and maybe some other goodies in 2-3 months so I am glad I purchased all this now since thatll be a lot easier in the future :)
EDIT: Also I just use sterile vials so all I do after the stuff is filtered is draw it up in a 60ml syringe and put a needle in the vial before dropping 10ml into each sealed vial, I know some people buy open vials and the flip tops which is a lot cooler but you'd have to sterilize the vials and I don't have an autoclave or an oven I could use. :(
Unfortunately, no. If you’re completely new to developing and haven’t yet done black and white, there’s a bit of an initial expense. I’m going to just list off everything I use for developing. It’s actually pretty compact, I’ve thrown it all in my car on impulse and developed C41 at friends’ houses a few times. I store it all in a small cooler which I also fill with water while developing. Since the developer needs to be 102°F with moderated precision for the duration of the developing time, it helps to have a larger body of water that won’t lose temperature as quickly. So first step for me is to fill this cooler slightly upwards of 102° to put the tank in while I’m not agitating. If you’ve got a cooler, great; if not:
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I think that’s about it! You can definitely go cheaper if you look around. The one thing I wouldn’t cheap out on though is the tank/reels. I started with a really cheap stainless steel setup. My reels came pretty bent up and took ages to load, like half an hour at times. I’d often have film stick to itself. I tried C41 with it one time and got blix everywhere, plus it was leaking in the cooler underwater. Everyone says Patterson is the way to go, and I haven’t tried much else but it’s served me well.
You don't actually need a dark room, it's a common misconception. You only need that if you plan to use an enlarger and make your own prints. I don't, but only due to lack of space in my apartment - I scan them in and order prints at Costco or Bartells like any normal digital scan.
If you get one of the Paterson Reel tanks like I have in that photo, you just need a changing bag. Put your 1-2 rolls of film, a bottle opener, a pair of scissors, and all the tank pieces in the bag. Seal it up, pop open the film canister with the bottle opener, then spool the film onto the reels. Put both reels back in the tank and seal it up. Everything else can be done in daylight. (you can skip the bag and do this in a pitch black room like your bathroom or a closet if you want. I prefer the bag- I can sit on the couch and watch TV while I spool rolls).
Then you're just dumping in chemicals on a timed schedule and then unspooling and hanging them to dry when complete. B&W is roughly 13 minutes a batch at diluted 1+1, or 6 minutes at stock (full strength) solution. Stock just uses it up twice as fast, but saves time.
B&W = developer (12-15 minutes for most films at 1+1 - check the massive dev chart -> stop bath (1 minute) -> fixer (1 minute) -> rinse (2-3 minutes) -> hang to dry (2ish hours or until totally dry).
Color = developer (6ish minutes) -> blix (2-3 minutes) -> rinse (2-3 minutes) -> stabilizer (1 minute) -> hang to dry (2ish hours).
I'm pulling those numbers from memory, but the unicolor kit has a good guide with it.
Stuff you need
For B&W film - you use the 1+1 developer one time (150ml per roll of film) and dump it when you finish. Which means that 1L D-76 pouch gives you 6 rolls of film. I buy mine in the 1G bags to mix up as they're cheaper, but you need a 1G plastic bottle to store it. For color film that kit does 8-9 rolls of film. The bigger 1G kits are more expensive and you can also buy the chemicals separately. Color is picky because you need to keep it at a constant 103F the whole time or risk ruining them, hence the bucket (or a bathtub). Color developer and all other chemicals you dump back into bottles and re-use until it's used up.
The color film process is actually really well documented in the booklet that comes with the unicolor kit.
If you ever want a hands on trial, I can do a video of the process or a demo at my apartment (my fiance thinks it looks like I'm doing crazy mad science stuff every time)
Check out /r/analog for weekly advice threads or some of these helpful links:
Cool add-ons to the Brooklyn Brew Shop 1G kits (which I liked):
http://www.amazon.com/Beer-Wort-Wine-Refractometer-Scale/dp/B006GG0TDK/ref=sr_1_3?ie=UTF8&qid=1398314617&sr=8-3&keywords=refractometer+dual+scale
http://www.amazon.com/Fermtech-MINI-Mini-Auto-Siphon/dp/B0064ODL1G/ref=sr_1_1?ie=UTF8&qid=1398314371&sr=8-1&keywords=mini+auto+siphon
http://www.amazon.com/Brewcraft-Fermtech-Plastic-Bottle-Filler/dp/B0064OEE9O/ref=sr_1_2?ie=UTF8&qid=1398314427&sr=8-2&keywords=bottling+wand
Starsan you don't have to rinse off, and it really helps you to be confident in your sanitizing abilities.
http://www.amazon.com/Five-Star-FS_SS_16-San--16oz/dp/B00FA86OZ2/ref=sr_1_3?ie=UTF8&qid=1398314939&sr=8-3&keywords=star+san
http://www.amazon.com/Bottle-Capper-Red-Baron-Homebrew/dp/B000Q6ARMY/ref=sr_1_1?ie=UTF8&qid=1398315533&sr=8-1&keywords=bottle+capper
http://www.homebrewtalk.com/f39/fermentation-temperature-control-440670/
Anyway, enough. Don't overthink it. Those kits are so freaking easy to do, my first batch turned out pretty drinkable despite some temperature screw ups during mashing.
You can watch homebrewfinds.com for deals on any items I listed, and you can even better, get them from your local home brew shop.
Note, if you think you want to do bigger batches later, the mini auto-siphon and any tiny kettles are going to be a waste of money... the refractometer, starsan, capper, etc. will still be quite useful.
I do agar with an SAB and a 23qt presto also.
These pyrex bottles are awesome for making agar https://www.amazon.com/gp/aw/d/B004XR5TUM/ref=ppx_yo_mob_b_inactive_ship_o0_img?ie=UTF8&psc=1
Look up recipes and find what you like. I use light malt extract ordered from amazon and the telephone brand agar https://www.amazon.com/gp/aw/d/B00D42CRIE/ref=ppx_yo_mob_b_inactive_ship_o0_img?ie=UTF8&psc=1
Agar is awesome but seems overwhelming at first but once you just get the stuff and try it you will love it.
You can order 500 ml pyrex jars on Amazon for 20 bucks. Pyrex makes universally durable borosilicate glass. This is the same thing you would get if you went through VWR or a lab supply company.
I would stay away from plastic- I don't know your application, but I would worry that plastic additives would leech into your solution. That depends on the type of plastic- look for the recycling label on the bottom, that will tell you what kind of plastic you have. I would think a thick HDPE jar would be satisfactory (nalgene makes lab-grade containers available on amazon as well), but pouring and cleaning plastic might be a little more trouble.
Here's the items I found- sorry for the formatting as I'm on mobile.
https://www.amazon.com/gp/aw/d/B004XR5TUM/
https://www.amazon.com/gp/aw/d/B000VKZX1U
http://www.ineos.com/globalassets/ineos-group/businesses/ineos-olefins-and-polymers-usa/products/technical-information--patents/ineos-hdpe-chemical-resistance-guide.pdf
To illustrate what BST said...
Bottle Top filter example: http://www.amazon.com/Corning-430049-Polystyrene-Membrane-Diameter/dp/B005J5BVBI
Media Bottle: http://www.amazon.com/PYREX-500mL-Round-Storage-Bottles/dp/B004XR5TUM/ref=sr_1_1?s=industrial&ie=UTF8&qid=1421326439&sr=1-1&keywords=borosilicate+media+bottle.+45mm&pebp=1421326442468&peasin=B004XR5TUM
For autoclaving, in college we would just cover the top of the bottle with a small piece of aluminum foil and secure it with autoclave tape (which will also indicate when a specific temp has been reached)
Tape: http://en.wikipedia.org/wiki/Autoclave_tape
Corning PYREX #1395-1L, 1000ml... https://www.amazon.com/dp/B07J6JP4D1?ref=ppx_pop_mob_ap_share. Hope that link works. These containers are great and safe. Yes I understand the reason for freezing, I just knew it wasn't part of KemFun tek. Looking forward to see a picture of what you pull tomorrow.
Nope, no problems or leaking at all. I like the bottle. It's a little pricey on Amazon right now but I got it a while back when it was like $10 less. These are probably just as good and much cheaper. I did buy them but haven't used them yet. However, comparing then side by side, the Pyrex bottle is much higher quality.
bottle top filters.
https://www.amazon.com/Whatman-10443421-Cellulose-
Regenerated-Bottletop/dp/B007GE9N3I/ref=sr_1_9?ie=UTF8&qid=1487620307&sr=8-9&keywords=whatman+filter
glass media bottle i use
https://www.amazon.com/PYREX-250mL-Round-Storage-Bottles/dp/B004XR5TIO/ref=sr_1_7?ie=UTF8&qid=1487620362&sr=8-7&keywords=glass+media+bottles
break bleeder
https://www.amazon.com/Mityvac-MV8000-Automotive-Test-Bleeding/dp/B00265M9SS/ref=sr_1_7?s=automotive&ie=UTF8&qid=1487620409&sr=1-7&keywords=brake+bleeder
This graduated 1L Pyrex jar is excellent. The cap is resistant to solvents and strong bases. It is also available in 2LGraduated 1L Pyrex Jar
I think this is the one I have.
Anything labeled GL-45 will work.
If you hit up amazon and search “glass media bottle” there are tons of good cheap options.
Archiving some of the best bottles also allows me to move on to opening new bottles sooner. It helps that I have an almost unlimited supply of these spiffy, never used, 250 mL storage bottles.
http://www.amazon.com/PYREX-250mL-Round-Storage-Bottles/dp/B004XR5TIO/ref=sr_1_12?ie=UTF8&qid=1449923824&sr=8-12&keywords=250+ml+glass+bottles
One of these
https://smile.amazon.com/Home-Brew-Ohio-W4-2A50-1Z5L-Bottle/dp/B007VFBLBE
I bought a 3/8" one but I wish I got the 1/2" just so it's faster.
Then you need a siphon
https://smile.amazon.com/Home-Brew-Ohio-Siphon-Multicolor/dp/B071NG9Y6S/
This is what I bought, but the price has been inflated since I bought it. I got mine for about $35.
They work well, though I suspect you can get them cheaper elsewhere. I wanted them quickly though, so I just went through Amazon.
I heard the “leaf of faith” director say that the locals steep it for over 24 hrs. I think it was on smart drug smarts podcast. Either way, that how i do it now.
For say 10 doses ill sous vide 50g of leaves in water at 180 degrees for 24hrs or more. Add water if you need to. Once done, ill strain then measure the remaining liquid and divide the total weight by 10 (doses) to get the portion amount. So if there’s 500mg of the extract, ill dose at 50g. Ill label the dose on the bottle and seal and leave in the fridge. I did a very large batch in july and its still works as well as the first dose.
I used these pyrex bottles in 500ml and 1000ml
Pyrex Slimline Media Bottle Easy Pour Corning 500ML Glass https://www.amazon.com/dp/B00QU9BVH6/ref=cm_sw_r_cp_api_HxHWBbN1S514X
I usually use Pyrex round media storage bottles because I can get them through a lab at work.
When I am making a huge starter though I use a 2L flask first and then a 5L flask.
I tried that it didn't work. If this makes any difference I'm using this media bottle https://www.amazon.com/gp/product/B01JM39858/ref=ppx_yo_dt_b_asin_title_o08_s00?ie=UTF8&psc=1
and this filter
https://www.medical-and-lab-supplies.com/500ml-whatman-zapcap-33-45mm-fitting-neck-0-22-micron-qty-1.html
Can I leave the brew in a beaker while my new pump arrives?